PRDX3 Polyclonal antibody

Name: PRDX3 Polyclonal antibody
Brand: Proteintech
SKU: 55087-1-AP

PRDX3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55087-1-AP

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, Peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

Validation Data Gallery

Applications:

AllWBIHC

WB analysis using 55087-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55087-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 55087-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55087-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 55087-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55087-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55087-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55087-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 55087-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 55087-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55087-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 55087-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 55087-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55087-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 55087-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 55087-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 55087-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PRDX3 antibody included
IHC staining of human liver cancer using 55087-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PRDX3 antibody included
IHC staining of human breast cancer using 55087-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55087-1-AP (Peroxiredoxin 3 antibody at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with PRDX3 antibody included
IHC staining of human breast cancer using 55087-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55087-1-AP (Peroxiredoxin 3 antibody at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with PRDX3 antibody included

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Cat No. 55087-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, HepG2 cells, MCF-7 cells, HeLa cells, mouse liver tissue, mouse brain tissue, THP-1 cells, rat liver tissue
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

55087-1-AP targets PRDX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	peroxiredoxin 3
Calculated molecular weight	28 kDa
Observed molecular weight	26-28 kDa
GenBank accession number	NM_006793
Gene symbol	PRDX3
Gene ID (NCBI)	10935
RRID	AB_10860268
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells(PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. This antibody is specific to PRDX3.

Protocols

Product Specific Protocols
WB protocol for PRDX3 antibody 55087-1-AP	Download protocol
IHC protocol for PRDX3 antibody 55087-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. Authors - Shun Nagashima View Article
rat	WB	Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. Authors - Bo Wei View Article
human	WB	J Cell Biochem Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells. Authors - Sulpizio Marilisa M View Article
rat	WB	Cardiovasc Toxicol Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function. Authors - Ying Li View Article
mouse	WB,IF	Respir Res Peroxiredoxin 3 has a crucial role in the macrophage polarization by regulating mitochondrial homeostasis Authors - Wenhui Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRDX3 Polyclonal antibody

PRDX3 Polyclonal Antibody for IHC, WB, ELISA

Validation Data Gallery

WB analysis using 55087-1-AP

WB analysis of MCF-7 using 55087-1-AP

WB analysis of HeLa using 55087-1-AP

WB analysis of MCF-7 using 55087-1-AP

WB analysis of mouse brain using 55087-1-AP

WB analysis of mouse brain using 55087-1-AP

WB analysis of HeLa using 55087-1-AP

WB analysis of mouse liver using 55087-1-AP

WB analysis of mouse liver using 55087-1-AP

WB analysis of mouse brain using 55087-1-AP

WB analysis of HEK-293 using 55087-1-AP

WB analysis of Jurkat using 55087-1-AP

WB analysis of HepG2 using 55087-1-AP

WB analysis of MCF-7 using 55087-1-AP

WB analysis of MCF-7 using 55087-1-AP

IHC staining of human liver cancer using 55087-1-AP

IHC staining of human liver cancer using 55087-1-AP

IHC staining of human breast cancer using 55087-1-AP

IHC staining of human breast cancer using 55087-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.

Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function.

Peroxiredoxin 3 has a crucial role in the macrophage polarization by regulating mitochondrial homeostasis