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  • KD/KO Validated

Liprin Alpha 1 Polyclonal antibody

Liprin Alpha 1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14175-1-AP

Synonyms

LAR interacting protein 1, LIP 1, LIP.1, LIP1, LIPRIN, Liprin alpha 1, PPFIA1



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPFIA1 Ready-To-Use IHC Kit. PPFIA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

14175-1-AP targets Liprin Alpha 1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Liprin Alpha 1 fusion protein Ag5381 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Calculated molecular weight 1202 aa, 136 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC034046
Gene symbol PPFIA1
Gene ID (NCBI) 8500
RRIDAB_2171592
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
IHC protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
IF protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
IP protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
FC protocol for Liprin Alpha 1 antibody 14175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity.

Authors - Xingqiao Xie
humanWB,IF

Nat Commun

PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.

Authors - Giulia Mana
  • KD Validated
mouseIF

Elife

Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion.

Authors - Dillon Jevon
humanIF

Stem Cells Transl Med

Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix.

Authors - Reena Singh
humanWB

J Cell Sci

Liprin-α1, ERC1 and LL5 identify a polarized, dynamic compartment implicated in cell migration.

Authors - Veronica Astro
  • KD Validated
humanIF

Open Biol

The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions

Authors - Xingchen Li