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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PLOD3 Monoclonal antibody

PLOD3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G12B8

Cat no : CL594-60058

Synonyms

LH3, Lysyl hydroxylase 3, PLOD3

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-60058

    IF Staining of HeLa using CL594-60058

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60058 (PLOD3 antibody) at dilution of 1:50.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60058-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60058 targets PLOD3 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PLOD3 fusion protein Ag1480 相同性解析による交差性が予測される生物種
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Calculated molecular weight 738 aa, 85 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC011674
Gene symbol PLOD3
Gene ID (NCBI) 8985
RRIDAB_2883412
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Protocols

Product Specific Protocols
IF protocol for CL594 PLOD3 antibody CL594-60058Download protocol
Standard Protocols
Click here to view our Standard Protocols