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PLAA Monoclonal antibody

PLAA Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

5C10G9

Cat no : 60244-1-Ig

Synonyms

DOA1, PLA2P, PLAA, PLAP

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 60244-1-Ig

    WB analysis of MCF-7 using 60244-1-Ig

    WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

  • WB analysis of ROS1728 using 60244-1-Ig

    WB analysis of ROS1728 using 60244-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 60244-1-Ig

    WB analysis of A431 using 60244-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 60244-1-Ig

    WB analysis of K-562 using 60244-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 60244-1-Ig

    WB analysis of MCF-7 using 60244-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 60244-1-Ig

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PLAA antibody included
  • IHC staining of human kidney using 60244-1-Ig

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PLAA antibody included

Tested Applications

Positive WB detected inROS1728 cells, K-562 cells, MCF-7 cells, A431 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLAA Ready-To-Use IHC Kit. PLAA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60244-1-Ig is PLAA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLAA fusion protein Ag3224 相同性解析による交差性が予測される生物種
Full Name phospholipase A2-activating protein
Calculated molecular weight 738 aa, 81 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC032551
Gene symbol PLAA
Gene ID (NCBI) 9373
RRIDAB_2881366
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

Protocols

Product Specific Protocols
WB protocol for PLAA antibody 60244-1-IgDownload protocol
IHC protocol for PLAA antibody 60244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols