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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26899

Synonyms

PKC ZETA, PKC2, PRKCZ, Protein kinase C zeta type, protein kinase C, zeta

Validation Data Gallery

Applications:
  • IF Staining of NIH/3T3 using CL488-26899

    IF Staining of NIH/3T3 using CL488-26899

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 PKC Zeta antibody (CL488-26899) at dilution of 1:200.

  • IF Staining of HeLa using CL488-26899

    IF Staining of HeLa using CL488-26899

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PKC Zeta antibody (CL488-26899) at dilution of 1:200.

Tested Applications

Positive IF detected inNIH/3T3 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 26899-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-26899 targets PKC Zeta in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Zeta fusion protein Ag25391 相同性解析による交差性が予測される生物種
Full Name protein kinase C, zeta
Calculated molecular weight 80 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC014270
Gene symbol PRKCZ
Gene ID (NCBI) 5590
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PKC Zeta antibody CL488-26899Download protocol
Standard Protocols
Click here to view our Standard Protocols