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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PICALM Monoclonal antibody

PICALM Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6G7

Cat no : 67564-1-Ig

Synonyms

CALM, CLTH, LAP, PICALM

Validation Data Gallery

Applications:
  • WB analysis using 67564-1-Ig

    WB analysis using 67564-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67564-1-Ig (PICALM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67564-1-Ig

    IHC staining of human lung cancer using 67564-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67564-1-Ig (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67564-1-Ig

    IF Staining of HeLa using 67564-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67564-1-Ig targets PICALM in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PICALM fusion protein Ag29512 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol binding clathrin assembly protein
Calculated molecular weight 71 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC064357
Gene symbol PICALM
Gene ID (NCBI) 8301
RRIDAB_2882778
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

Protocols

Product Specific Protocols
WB protocol for PICALM antibody 67564-1-IgDownload protocol
IHC protocol for PICALM antibody 67564-1-IgDownload protocol
IF protocol for PICALM antibody 67564-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols