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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PICALM Monoclonal antibody (67564-1-Ig)

    WB analysis using 67564-1-Ig (same clone as 67564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67564-1-Ig (PICALM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PICALM Monoclonal antibody (67564-1-Ig)

    IHC staining of human lung cancer using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67564-1-Ig (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PICALM Monoclonal antibody (67564-1-Ig)

    IF Staining of HeLa using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PICALM Monoclonal antibody (67564-1-Ig)

    IF Staining of HepG2 using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

PICALM Monoclonal antibody, PBS Only

PICALM Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67564-1-PBS
Clone No.1F6G7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1F6G7, CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67564-1-PBS targets PICALM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29512

Product name: Recombinant human PICALM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-356 aa of BC064357

Sequence: MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTT

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol binding clathrin assembly protein
Calculated molecular weight 71 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC064357
Gene Symbol PICALM
Gene ID (NCBI) 8301
RRIDAB_2882778
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13492
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

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