Prohibitin Monoclonal antibody
Prohibitin Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4D3G5
Cat no : 60092-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
60092-1-Ig targets Prohibitin in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, rat samples.
| Tested Reactivity | human, pig, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Prohibitin fusion protein Ag1240 相同性解析による交差性が予測される生物種 |
| Full Name | prohibitin |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC013401 |
| Gene symbol | PHB |
| Gene ID (NCBI) | 5245 |
| RRID | AB_2252403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Prohibitin antibody 60092-1-Ig | Download protocol |
| IHC protocol for Prohibitin antibody 60092-1-Ig | Download protocol |
| IF protocol for Prohibitin antibody 60092-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Chron Obstruct Pulmon Dis Prohibitin Protects Pulmonary Microvascular Endothelial Cells Against Cigarette Smoke Extract-Induced Cell Apoptosis and Inflammation. | ||
Oncotarget Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells. |
