Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells, HepG2 cells, pig liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human cervical cancer tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
60249-1-Ig targets PGRMC2 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20077 Product name: Recombinant human PGRMC2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 55-145 aa of BC016692 Sequence: LLNVALVALVLLGAYRLWVRWGRRGLGAGAGAGEESPATSLPRMKKRDFSLEQLRQYDGSRNPRILLAVNGKVFDVTKGSKFYGPAGPYGI 相同性解析による交差性が予測される生物種 |
| Full Name | progesterone receptor membrane component 2 |
| Calculated molecular weight | 223 aa, 24 kDa |
| Observed molecular weight | 24 kDa, 28 kDa |
| GenBank accession number | BC016692 |
| Gene Symbol | PGRMC2 |
| Gene ID (NCBI) | 10424 |
| RRID | AB_2881370 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15173 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PGRMC2 antibody 60249-1-Ig | Download protocol |
| IHC protocol for PGRMC2 antibody 60249-1-Ig | Download protocol |
| WB protocol for PGRMC2 antibody 60249-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Sci Topology surveillance of the lanosterol demethylase CYP51A1 by Signal Peptide Peptidase | ||
Biol Chem A progesterone receptor membrane component 1 antagonist induces large vesicles independent of progesterone receptor membrane component 1 expression. | ||
Int J Clin Exp Pathol Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.
| ||
Ibrain Progesterone receptor membrane component 2 regulates the neuronal activity and participates in epileptic seizures in experimental mice |
