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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PER2 Polyclonal antibody

PER2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29954-1-AP

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)

Validation Data Gallery

Applications:
  • WB analysis using 29954-1-AP

    WB analysis using 29954-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29954-1-AP (PER2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MDA-MB-231 using 29954-1-AP

    IF Staining of MDA-MB-231 using 29954-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using PER2 antibody (29954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IF detected inMDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29954-1-AP targets PER2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PER2 fusion protein Ag31471 相同性解析による交差性が予測される生物種
Full Name period homolog 2 (Drosophila)
Calculated molecular weight137KD
Observed molecular weight45 kDa
GenBank accession numberNM_022817
Gene symbol PER2
Gene ID (NCBI) 8864
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774). 29954-1-AP detected molecular weight of 45 kDa.

Protocols

Product Specific Protocols
WB protocol for PER2 antibody 29954-1-APDownload protocol
IF protocol for PER2 antibody 29954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols