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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEBP1 Monoclonal antibody

PEBP1 Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2D4D11

Cat no : 66438-1-Ig

Synonyms

HCNP, HCNPpp, Neuropolypeptide h3, PBP, PEBP, PEBP 1, PEBP1, Prostatic binding protein, Raf kinase inhibitor protein, RKIP

Validation Data Gallery

Applications:
  • WB analysis of fetal human brain using 66438-1-Ig

    WB analysis of fetal human brain using 66438-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 66438-1-Ig

    WB analysis of rat brain using 66438-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Neuro-2a using 66438-1-Ig

    WB analysis of Neuro-2a using 66438-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 66438-1-Ig

    WB analysis of PC-12 using 66438-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66438-1-Ig

    IHC staining of human prostate cancer using 66438-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 10x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

    Ready-to-use IHC kits available with PEBP1 antibody included
  • IHC staining of human prostate cancer using 66438-1-Ig

    IHC staining of human prostate cancer using 66438-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 40x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

    Ready-to-use IHC kits available with PEBP1 antibody included
  • FC experiment of PC-12 using 66438-1-Ig

    FC experiment of PC-12 using 66438-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human PEBP1 (66438-1-Ig, Clone:2D4D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected infetal human brain tissue, Neuro-2a cells, PC-12 cells, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy PEBP1 Ready-To-Use IHC Kit. PEBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66438-1-Ig targets PEBP1 in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEBP1 fusion protein Ag0864 相同性解析による交差性が予測される生物種
Full Name phosphatidylethanolamine binding protein 1
Calculated molecular weight 21 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC008714
Gene symbol PEBP1
Gene ID (NCBI) 5037
RRIDAB_2881808
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.

Protocols

Product Specific Protocols
WB protocol for PEBP1 antibody 66438-1-IgDownload protocol
IHC protocol for PEBP1 antibody 66438-1-IgDownload protocol
FC protocol for PEBP1 antibody 66438-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols