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- KD/KO Validated
PDLIM7 Polyclonal antibody
PDLIM7 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17080-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human liver tissue, human kidney tissue, human ovary tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Product Information
17080-1-AP targets PDLIM7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDLIM7 fusion protein Ag1334 相同性解析による交差性が予測される生物種 |
| Full Name | PDZ and LIM domain 7 (enigma) |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC014521 |
| Gene symbol | PDLIM7 |
| Gene ID (NCBI) | 9260 |
| RRID | AB_2161788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDLIM7 antibody 17080-1-AP | Download protocol |
| IHC protocol for PDLIM7 antibody 17080-1-AP | Download protocol |
| IP protocol for PDLIM7 antibody 17080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
