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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDHB Monoclonal antibody

PDHB Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A7C10

Cat no : 68238-1-Ig

Synonyms

DKFZp564K0164, PDHB, PDHE1 B, PHE1B

Validation Data Gallery

Applications:
  • WB analysis using 68238-1-Ig

    WB analysis using 68238-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68238-1-Ig

    IF Staining of HeLa using 68238-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, mouse heart tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68238-1-Ig targets PDHB in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDHB fusion protein Ag6857 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) beta
Calculated molecular weight 39 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000439
Gene symbol PDHB
Gene ID (NCBI) 5162
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

Protocols

Product Specific Protocols
WB protocol for PDHB antibody 68238-1-IgDownload protocol
IF protocol for PDHB antibody 68238-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols