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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PCK2 Monoclonal antibody

PCK2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B3F1

Cat no : CL488-67676

Synonyms

PCK2, PEPCK, PEPCK M, PEPCK2

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-67676

    FC experiment of HeLa using CL488-67676

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PCK2 (CL488-67676, Clone:1B3F1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCK2 Ready-To-Use IHC Kit. PCK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67676-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67676 targets PCK2 in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCK2 fusion protein Ag7021 相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Calculated molecular weight 71 kDa
Observed molecular weight68 kDa
GenBank accession numberBC001454
Gene symbol PCK2
Gene ID (NCBI) 5106
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 PCK2 antibody CL488-67676Download protocol
Standard Protocols
Click here to view our Standard Protocols