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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PCK1 Monoclonal antibody

PCK1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig, human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F11B5

Cat no : CL488-66862

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-66862

    IF Staining of HeLa using CL488-66862

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PCK1 antibody (CL488-66862, Clone: 2F11B5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66862-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66862 targets PCK1 in IF applications and shows reactivity with mouse, rat, pig, human samples.

Tested Reactivity mouse, rat, pig, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCK1 fusion protein Ag9720 相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated molecular weight 622 aa, 69 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC023978
Gene symbol PCK1
Gene ID (NCBI) 5105
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PCK1 antibody CL488-66862Download protocol
Standard Protocols
Click here to view our Standard Protocols