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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCK1 Monoclonal antibody

PCK1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11B5

Cat no : 66862-1-Ig

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

Validation Data Gallery

Applications:
  • WB analysis using 66862-1-Ig

    WB analysis using 66862-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 66862-1-Ig

    IHC staining of mouse kidney using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of mouse kidney using 66862-1-Ig

    IHC staining of mouse kidney using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of human colon using 66862-1-Ig

    IHC staining of human colon using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of human colon using 66862-1-Ig

    IHC staining of human colon using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of human kidney using 66862-1-Ig

    IHC staining of human kidney using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of human kidney using 66862-1-Ig

    IHC staining of human kidney using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included
  • IF Staining of HeLa using 66862-1-Ig

    IF Staining of HeLa using 66862-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig kidney tissue, pig liver tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66862-1-Ig targets PCK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCK1 fusion protein Ag9720 相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated molecular weight 622 aa, 69 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC023978
Gene symbol PCK1
Gene ID (NCBI) 5105
RRIDAB_2882200
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Protocols

Product Specific Protocols
WB protocol for PCK1 antibody 66862-1-IgDownload protocol
IHC protocol for PCK1 antibody 66862-1-IgDownload protocol
IF protocol for PCK1 antibody 66862-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols