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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PARD3 Polyclonal antibody

PARD3 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11085

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

Validation Data Gallery

Applications:
  • IF Staining of mouse colon using CL488-11085

    IF Staining of mouse colon using CL488-11085

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 PARD3 antibody (CL488-11085) at dilution of 1:100.

  • IF Staining of mouse colon using CL488-11085

    IF Staining of mouse colon using CL488-11085

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 PARD3 antibody (CL488-11085) at dilution of 1:100.

Tested Applications

Positive IF detected inmouse colon tissue
For other applications, we recommend the unconjugated version of this antibody, 11085-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-11085 targets PARD3 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARD3 fusion protein Ag1565 相同性解析による交差性が予測される生物種
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Calculated molecular weight 151 kDa
Observed molecular weight 180 kDa, 140-150 kDa, 100 kDa
GenBank accession numberBC011711
Gene symbol PARD3
Gene ID (NCBI) 56288
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PARD3 antibody CL488-11085Download protocol
Standard Protocols
Click here to view our Standard Protocols