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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PARD3 Monoclonal antibody

PARD3 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2B2

Cat no : 66968-1-Ig

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 66968-1-Ig

    WB analysis of MCF-7 using 66968-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66968-1-Ig

    WB analysis of HeLa using 66968-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66968-1-Ig

    WB analysis of HEK-293 using 66968-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 66968-1-Ig

    WB analysis of A431 using 66968-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66968-1-Ig

    WB analysis of HepG2 using 66968-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66968-1-Ig

    IF Staining of MCF-7 using 66968-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PARD3 antibody (66968-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of MCF-7 using 66968-1-Ig

    FC experiment of MCF-7 using 66968-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PARD3 (66968-1-Ig, Clone:1D2B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, A431 cells, HepG2 cells
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66968-1-Ig targets PARD3 in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARD3 fusion protein Ag7656 相同性解析による交差性が予測される生物種
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Calculated molecular weight 151 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC011711
Gene symbol PARD3
Gene ID (NCBI) 56288
RRIDAB_2882290
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.

Protocols

Product Specific Protocols
WB protocol for PARD3 antibody 66968-1-IgDownload protocol
IF protocol for PARD3 antibody 66968-1-IgDownload protocol
FC protocol for PARD3 antibody 66968-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols