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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Occludin Recombinant antibody

Occludin Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

8B2

Cat no : CL488-80545

Synonyms

occludin, OCLN

Validation Data Gallery

Applications:
  • IF Staining of MCF-7 using CL488-80545

    IF Staining of MCF-7 using CL488-80545

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 Occludin antibody (CL488-80545, Clone: 8B2 ) at dilution of 1:200.

Tested Applications

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 80545-1-PBS

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-80545 targets Occludin in IF applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity Human, Mouse, Pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Occludin fusion protein Ag26173 相同性解析による交差性が予測される生物種
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC029886
Gene symbol OCLN
Gene ID (NCBI) 4950
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Occludin antibody CL488-80545Download protocol
Standard Protocols
Click here to view our Standard Protocols