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CoraLite® Plus 488-conjugated OTX2 Polyclonal antibody
OTX2 Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-13497
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse embryo tissue |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-13497 targets OTX2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | OTX2 fusion protein Ag4323 相同性解析による交差性が予測される生物種 |
Full Name | orthodenticle homeobox 2 |
Calculated molecular weight | 297 aa, 32 kDa |
Observed molecular weight | 32 kDa |
GenBank accession number | BC032579 |
Gene symbol | OTX2 |
Gene ID (NCBI) | 5015 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 OTX2 antibody CL488-13497 | Download protocol |
FC protocol for CL Plus 488 OTX2 antibody CL488-13497 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |