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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated OTX2 Polyclonal antibody

OTX2 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13497

Synonyms

Homeobox protein OTX2, MCOPS5, orthodenticle homeobox 2, Orthodenticle homolog 2, OTX2

Validation Data Gallery

Applications:
  • IF Staining of mouse embryo using CL488-13497

    IF Staining of mouse embryo using CL488-13497

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using CoraLite® Plus 488 OTX2 antibody (CL488-13497) at dilution of 1:200.

  • IF Staining of mouse embryo using CL488-13497

    IF Staining of mouse embryo using CL488-13497

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using CoraLite® Plus 488 OTX2 antibody (CL488-13497) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-13497

    FC experiment of HepG2 using CL488-13497

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human OTX2 (CL488-13497) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inmouse embryo tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13497-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-13497 targets OTX2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTX2 fusion protein Ag4323 相同性解析による交差性が予測される生物種
Full Name orthodenticle homeobox 2
Calculated molecular weight 297 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC032579
Gene symbol OTX2
Gene ID (NCBI) 5015
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 OTX2 antibody CL488-13497Download protocol
FC protocol for CL Plus 488 OTX2 antibody CL488-13497Download protocol
Standard Protocols
Click here to view our Standard Protocols