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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OTUB1 Monoclonal antibody

OTUB1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F7F8

Cat no : 68489-1-Ig

Synonyms

Deubiquitinating enzyme OTUB1, FLJ20113, FLJ40710, hOTU1, HSPC263, OTB1, OTU1, OTUB1, Otubain 1, Ubiquitin thioesterase OTUB1

Validation Data Gallery

Applications:
  • WB analysis using 68489-1-Ig

    WB analysis using 68489-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68489-1-Ig (OTUB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68489-1-Ig targets OTUB1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen OTUB1 fusion protein Ag33132 相同性解析による交差性が予測される生物種
Full Name OTU domain, ubiquitin aldehyde binding 1
Calculated molecular weight 31 kDa and 35 kDa
Observed molecular weight31-35 kDa
GenBank accession numberBC007519
Gene symbol OTUB1
Gene ID (NCBI) 55611
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for OTUB1 antibody 68489-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols