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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OTC Monoclonal antibody

OTC Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11C7

Cat no : 67553-1-Ig

Synonyms

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

Validation Data Gallery

Applications:
  • WB analysis using 67553-1-Ig

    WB analysis using 67553-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67553-1-Ig (OTC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67553-1-Ig

    IHC staining of human liver using 67553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with OTC antibody included
  • IHC staining of human liver cancer using 67553-1-Ig

    IHC staining of human liver cancer using 67553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with OTC antibody included
  • IHC staining of rat colon using 67553-1-Ig

    IHC staining of rat colon using 67553-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with OTC antibody included

Tested Applications

Positive WB detected inpig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy OTC Ready-To-Use IHC Kit. OTC primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Sample-dependent, check data in validation data gallery

Product Information

67553-1-Ig targets OTC in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen OTC fusion protein Ag24186 相同性解析による交差性が予測される生物種
Full Name ornithine carbamoyltransferase
Calculated molecular weight 354 aa, 40 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC074745
Gene symbol OTC
Gene ID (NCBI) 5009
RRIDAB_2882767
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.

Protocols

Product Specific Protocols
WB protocol for OTC antibody 67553-1-IgDownload protocol
IHC protocol for OTC antibody 67553-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols