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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-19483

Synonyms

FLJ21841, Nbla00170, NES, nestin

Validation Data Gallery

Applications:
  • IF Staining of rat brain using CL594-19483

    IF Staining of rat brain using CL594-19483

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 Nestin antibody (CL594-19483) at dilution of 1:200. DAPI (blue).

  • IF Staining of human kidney using CL594-19483

    IF Staining of human kidney using CL594-19483

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 Nestin antibody (CL594-19483) at dilution of 1:200.

Tested Applications

Positive IF detected inrat brain tissue, human kidney tissue
For other applications, we recommend the unconjugated version of this antibody, 19483-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-19483 targets Nestin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight 170-180 kDa
GenBank accession numberNM_006617
Gene symbol NES
Gene ID (NCBI) 10763
RRIDAB_2919852
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Protocols

Product Specific Protocols
IF protocol for CL594 Nestin antibody CL594-19483Download protocol
Standard Protocols
Click here to view our Standard Protocols