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Species Reactivity

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  • Featured Product
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NSF Monoclonal antibody

NSF Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rabbit, Rat, Mouse, Chicken

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B6H6

Cat no : 68061-1-Ig

Synonyms

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

Validation Data Gallery

Applications:
  • WB analysis using 68061-1-Ig

    WB analysis using 68061-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 68061-1-Ig

    WB analysis of PC-12 using 68061-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 68061-1-Ig

    WB analysis of SH-SY5Y using 68061-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68061-1-Ig

    WB analysis of HEK-293 using 68061-1-Ig

    WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells.

  • IF Staining of mouse brain using 68061-1-Ig

    IF Staining of mouse brain using 68061-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, SH-SY5Y cells, PC-12 cells, HEK-293 cells, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue, Chicken brain tissue
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

68061-1-Ig targets NSF in WB, IF, ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse, Chicken samples.

Tested Reactivity Human, Pig, Rabbit, Rat, Mouse, Chicken
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NSF fusion protein Ag15422 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor
Calculated molecular weight 744 aa, 83 kDa
Observed molecular weight70-83 kDa
GenBank accession numberBC030613
Gene symbol NSF
Gene ID (NCBI) 4905
RRIDAB_2918802
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NSF antibody 68061-1-IgDownload protocol
IF protocol for NSF antibody 68061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols