CoraLite® Plus 488-conjugated NNT Monoclonal antibody
NNT Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat, Rabbit, Pig
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F5H6
Cat no : CL488-68194
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-68194 targets NNT in IF applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Rabbit, Pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NNT fusion protein Ag4259 相同性解析による交差性が予測される生物種 |
| Full Name | nicotinamide nucleotide transhydrogenase |
| Calculated molecular weight | 1085 aa, 114 kDa |
| Observed molecular weight | 114 kDa |
| GenBank accession number | BC032370 |
| Gene symbol | NNT |
| Gene ID (NCBI) | 23530 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NNT antibody CL488-68194 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
