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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NNT Monoclonal antibody

NNT Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F5H6

Cat no : 68194-1-Ig

Synonyms

NNT

Validation Data Gallery

Applications:
  • WB analysis of LNCaP using 68194-1-Ig

    WB analysis of LNCaP using 68194-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68194-1-Ig

    WB analysis using 68194-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68194-1-Ig

    WB analysis using 68194-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 68194-1-Ig

    IP experiment of HepG2 using 68194-1-Ig

    IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug.

  • IHC staining of mouse heart using 68194-1-Ig

    IHC staining of mouse heart using 68194-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NNT antibody included
  • IHC staining of human liver using 68194-1-Ig

    IHC staining of human liver using 68194-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NNT antibody included
  • IHC staining of human liver using 68194-1-Ig

    IHC staining of human liver using 68194-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NNT antibody included
  • IHC staining of mouse heart using 68194-1-Ig

    IHC staining of mouse heart using 68194-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NNT antibody included
  • IF Staining of HeLa using 68194-1-Ig

    IF Staining of HeLa using 68194-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 68194-1-Ig

    IF Staining of HeLa using 68194-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig heart tissue, LNCaP cells, HeLa cells, Jurkat cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat liver tissue, mouse liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NNT Ready-To-Use IHC Kit. NNT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68194-1-Ig targets NNT in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NNT fusion protein Ag4259 相同性解析による交差性が予測される生物種
Full Name nicotinamide nucleotide transhydrogenase
Calculated molecular weight 1085 aa, 114 kDa
Observed molecular weight114 kDa
GenBank accession numberBC032370
Gene symbol NNT
Gene ID (NCBI) 23530
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

Protocols

Product Specific Protocols
WB protocol for NNT antibody 68194-1-IgDownload protocol
IHC protocol for NNT antibody 68194-1-IgDownload protocol
IF protocol for NNT antibody 68194-1-IgDownload protocol
IP protocol for NNT antibody 68194-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols