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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NFIA Monoclonal antibody

NFIA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A6D7

Cat no : CL488-66999

Synonyms

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-66999

    FC experiment of HeLa using CL488-66999

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NFIA (CL488-66999, Clone:3A6D7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NFIA Ready-To-Use IHC Kit. NFIA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66999-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66999 targets NFIA in FC (Intra) applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

Tested Reactivity Human, rat, mouse, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFIA fusion protein Ag17873 相同性解析による交差性が予測される生物種
Full Name nuclear factor I/A
Calculated molecular weight 498 aa, 55 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC022264
Gene symbol NFIA
Gene ID (NCBI) 4774
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 NFIA antibody CL488-66999Download protocol
Standard Protocols
Click here to view our Standard Protocols