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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NECAB1 Polyclonal antibody

NECAB1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16477-1-AP

Synonyms

EFCBP1, NECAB1, STIP 1

Validation Data Gallery

Applications:
  • IHC staining of mouse brain using 16477-1-AP

    IHC staining of mouse brain using 16477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16477-1-AP (NECAB1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16477-1-AP

    IHC staining of mouse brain using 16477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16477-1-AP (NECAB1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 16477-1-AP

    IF Staining of mouse brain using 16477-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NECAB1 antibody (16477-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16477-1-AP targets NECAB1 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NECAB1 fusion protein Ag9591 相同性解析による交差性が予測される生物種
Full Name N-terminal EF-hand calcium binding protein 1
Calculated molecular weight 351 aa, 40 kDa
GenBank accession numberBC016340
Gene symbol NECAB1
Gene ID (NCBI) 64168
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NECAB1, also named as EFCBP1, is a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing EF-hand and antibiotic biosynthesis monooxygenase domains Short Communication.

Protocols

Product Specific Protocols
IHC protocol for NECAB1 antibody 16477-1-APDownload protocol
IF protocol for NECAB1 antibody 16477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols