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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFV1 Monoclonal antibody

NDUFV1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rabbit, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E9D4

Cat no : 68144-1-Ig

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

Validation Data Gallery

Applications:
  • WB analysis using 68144-1-Ig

    WB analysis using 68144-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68144-1-Ig

    WB analysis using 68144-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68144-1-Ig

    WB analysis using 68144-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 68144-1-Ig

    WB analysis of 4T1 using 68144-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68144-1-Ig

    IHC staining of human lung cancer using 68144-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFV1 antibody included
  • IHC staining of human lung cancer using 68144-1-Ig

    IHC staining of human lung cancer using 68144-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFV1 antibody included
  • IHC staining of mouse skeletal muscle using 68144-1-Ig

    IHC staining of mouse skeletal muscle using 68144-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFV1 antibody included
  • IHC staining of mouse skeletal muscle using 68144-1-Ig

    IHC staining of mouse skeletal muscle using 68144-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFV1 antibody included

Tested Applications

Positive WB detected inPC-12 cells, A549 cells, 4T1 cells, pig heart tissue, HeLa cells, HEK-293 cells, H9C2 cells, rabbit heart tissue
Positive IHC detected inhuman lung cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDUFV1 Ready-To-Use IHC Kit. NDUFV1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

68144-1-Ig targets NDUFV1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.

Tested Reactivity Human, pig, rabbit, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDUFV1 fusion protein Ag32659 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Calculated molecular weight 51 kDa
Observed molecular weight45 kDa
GenBank accession numberBC015645
Gene symbol NDUFV1
Gene ID (NCBI) 4723
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

Protocols

Product Specific Protocols
WB protocol for NDUFV1 antibody 68144-1-IgDownload protocol
IHC protocol for NDUFV1 antibody 68144-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols