CoraLite® Plus 488-conjugated NCALD Monoclonal antibody
NCALD Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6B4E10
Cat no : CL488-66088
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66088-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
The immunogen of CL488-66088 is NCALD Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NCALD fusion protein Ag18760 相同性解析による交差性が予測される生物種 |
| Full Name | neurocalcin delta |
| Calculated molecular weight | 193 aa, 22 kDa |
| GenBank accession number | BC036098 |
| Gene symbol | NCALD |
| Gene ID (NCBI) | 83988 |
| RRID | AB_2919285 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NCALD antibody CL488-66088 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
