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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Napsin A Monoclonal antibody

Napsin A Monoclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7F2

Cat no : 60259-2-Ig

Synonyms

Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02

Validation Data Gallery

Applications:
  • IHC staining of human lung cancer using 60259-2-Ig

    IHC staining of human lung cancer using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human lung cancer using 60259-2-Ig

    IHC staining of human lung cancer using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human renal cell carcinoma using 60259-2-Ig

    IHC staining of human renal cell carcinoma using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human renal cell carcinoma using 60259-2-Ig

    IHC staining of human renal cell carcinoma using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human kidney using 60259-2-Ig

    IHC staining of human kidney using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human kidney using 60259-2-Ig

    IHC staining of human kidney using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IF Staining of HUVEC using 60259-2-Ig

    IF Staining of HUVEC using 60259-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (60259-2-Ig, Clone: 1H7F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of A549 using 60259-2-Ig

    FC experiment of A549 using 60259-2-Ig

    1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HUVEC using 60259-2-Ig

    FC experiment of HUVEC using 60259-2-Ig

    1X10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman lung cancer tissue, human kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells
Positive FC detected inA549 cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy Napsin A Ready-To-Use IHC Kit. Napsin A primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:10000-1:60000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

60259-2-Ig targets Napsin A in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Napsin A fusion protein Ag9721 相同性解析による交差性が予測される生物種
Full Name napsin A aspartic peptidase
Calculated molecular weight 420 aa, 45 kDa
GenBank accession numberBC017842
Gene symbol NAPSA
Gene ID (NCBI) 9476
RRIDAB_2881381
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

Protocols

Product Specific Protocols
IHC protocol for Napsin A antibody 60259-2-IgDownload protocol
IF protocol for Napsin A antibody 60259-2-IgDownload protocol
FC protocol for Napsin A antibody 60259-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells.

Authors - Yanyun Du
View Article