• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human lung cancer using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human lung cancer using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human renal cell carcinoma using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human renal cell carcinoma using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Napsin A Monoclonal antibody (60259-2-Ig)

    IF Staining of HUVEC using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (60259-2-Ig, Clone: 1H7F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using Napsin A Monoclonal antibody (60259-2-Ig)

    FC experiment of A549 using 60259-2-Ig (same clone as 60259-2-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

Napsin A Monoclonal antibody, PBS Only

Napsin A Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 60259-2-PBS
Clone No.1H7F2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

NAPSA, 1H7F2, Asp 4, ASP4, Aspartyl protease 4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60259-2-PBS targets Napsin A in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9721

Product name: Recombinant human NAPSA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-420 aa of BC017842

Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG

相同性解析による交差性が予測される生物種
Full Name napsin A aspartic peptidase
Calculated molecular weight 420 aa, 45 kDa
GenBank accession numberBC017842
Gene Symbol Napsin A
Gene ID (NCBI) 9476
RRIDAB_2881381
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO96009
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

||
New chat Able
正在加载，请稍候...