Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata and More (1)

Applications

WB, RIP, IP, IHC, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

1F8D3

Cat no : 66360-1-Ig

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control


"Mouse IgG1 isotype control Antibodies" Comparison

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Applications:
  • WB analysis using 66360-1-Ig

    WB analysis using 66360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66360-1-Ig

    WB analysis using 66360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 66360-1-Ig

    FC experiment of K-562 using 66360-1-Ig

    1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.


Tested Applications

Positive WB detected in
Positive FC detected in

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

66360-1-Ig targets Mouse IgG1 isotype control in WB, RIP, IP, IHC, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Mouse IgG1 isotype control
Gene symbol
Gene ID (NCBI)
RRIDAB_2827991
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
FC protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

FASEB J

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection

Authors - Chujie Ding
IP

Exp Cell Res

CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.

Authors - Yuting Yang
IHC

Am J Rhinol Allergy

Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.

Authors - Tomohiro Kawasumi
RIP

Cell Biosci

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression

Authors - Liang Chen

Stem Cell Res Ther

Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models

Authors - Han Li
IP

Acta Biochim Biophys Sin (Shanghai)

PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation

Authors - Gongyu Fu