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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse IgG1 isotype control Monoclonal antibody, PBS Only

Mouse IgG1 isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F8D3

Cat no : 66360-1-PBS

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

Validation Data Gallery

Applications:
  • WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

    WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

  • WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

    WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 66360-1-Ig (same clone as 66360-1-PBS)

    FC experiment of K-562 using 66360-1-Ig (same clone as 66360-1-PBS)

    1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66360-1-PBS targets Mouse IgG1 isotype control in WB, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Mouse IgG1 isotype control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).