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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mmp9 Polyclonal antibody

Mmp9 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30592-1-AP

Synonyms

AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9, MMP 9, Mmp9, pro MMP 9

Validation Data Gallery

Applications:
  • WB analysis using 30592-1-AP

    WB analysis using 30592-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30592-1-AP (Mmp9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30592-1-AP

    IF Staining of A431 using 30592-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 30592-1-AP

    IF Staining of U2OS using 30592-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse lung tissue, mouse spleen tissue
Positive IF detected inU2OS cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

30592-1-AP targets Mmp9 in WB, IF, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 9
Calculated molecular weight81kd
Observed molecular weight80-100 kDa
GenBank accession numberNM_013599
Gene symbol Mmp9
Gene ID (NCBI) 17395
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).

Protocols

Product Specific Protocols
WB protocol for Mmp9 antibody 30592-1-APDownload protocol
IF protocol for Mmp9 antibody 30592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols