Mlkl Polyclonal antibody
Mlkl Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Mouse and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 30145-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | 4T1 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
30145-1-AP targets Mlkl in WB, ELISA applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mlkl fusion protein Ag32514 相同性解析による交差性が予測される生物種 |
| Full Name | mixed lineage kinase domain-like |
| Calculated molecular weight | 54kd |
| Observed molecular weight | 54 kDa |
| GenBank accession number | NM_001310613 |
| Gene symbol | Mlkl |
| Gene ID (NCBI) | 74568 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Mlkl antibody 30145-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
