CoraLite®594-conjugated MYPT1 Monoclonal antibody
MYPT1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG3
Reactivity
Human, rat, mouse
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2A1A9
Cat no : CL594-66506
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL594-66506 targets MYPT1 in IF applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MYPT1 fusion protein Ag17496 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 12A |
| Calculated molecular weight | 1030 aa, 115 kDa |
| Observed molecular weight | 115 kDa |
| GenBank accession number | BC111752 |
| Gene symbol | PPP1R12A |
| Gene ID (NCBI) | 4659 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MYPT1 antibody CL594-66506 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
