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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTHFD2 Monoclonal antibody

MTHFD2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B4B6

Cat no : 68524-1-Ig

Synonyms

MTHFD2

Validation Data Gallery

Applications:
  • WB analysis using 68524-1-Ig

    WB analysis using 68524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 68524-1-Ig

    IHC staining of mouse kidney using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 68524-1-Ig

    IHC staining of human lung cancer using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 68524-1-Ig

    IHC staining of human lung cancer using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 68524-1-Ig

    IHC staining of mouse kidney using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, LNCaP cells, HCT 116 cells, HeLa cells, HepG2 cells, K-562 cells, U2OS cellls
Positive IHC detected inhuman lung cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

68524-1-Ig targets MTHFD2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MTHFD2 fusion protein Ag33605 相同性解析による交差性が予測される生物種
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Calculated molecular weight 350 aa, 38 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC017054
Gene symbol MTHFD2
Gene ID (NCBI) 10797
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

Protocols

Product Specific Protocols
WB protocol for MTHFD2 antibody 68524-1-IgDownload protocol
IHC protocol for MTHFD2 antibody 68524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols