CoraLite® Plus 488-conjugated MTHFD1 Monoclonal antibody
MTHFD1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B1G3
Cat no : CL488-67670
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67670-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67670 targets MTHFD1 in IF applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MTHFD1 fusion protein Ag24553 相同性解析による交差性が予測される生物種 |
| Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase |
| Calculated molecular weight | 101 kDa |
| Observed molecular weight | 101 kDa |
| GenBank accession number | BC009806 |
| Gene symbol | MTHFD1 |
| Gene ID (NCBI) | 4522 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MTHFD1 antibody CL488-67670 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
