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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTHFD1 Monoclonal antibody (67670-1-Ig)

    WB analysis using 67670-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67670-1-Ig (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IHC staining of human colon cancer using 67670-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IHC staining of human colon cancer using 67670-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IHC staining of human liver cancer using 67670-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IHC staining of human liver cancer using 67670-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IHC staining of human liver cancer using 67670-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IHC staining of human liver cancer using 67670-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IF Staining of HEK-293 using 67670-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MTHFD1 Monoclonal antibody (67670-1-Ig)

    IF Staining of HEK-293 using 67670-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • Featured Product
  • KD/KO Validated

MTHFD1 Monoclonal antibody

MTHFD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67670-1-Ig
Clone No.2B1G3
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, A431 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67670-1-Ig targets MTHFD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MTHFD1 fusion protein Ag24553 相同性解析による交差性が予測される生物種
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC009806
Gene Symbol MTHFD1
Gene ID (NCBI) 4522
RRIDAB_2882866
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Protocols

Product Specific Protocols
WB protocol for MTHFD1 antibody 67670-1-IgDownload protocol
IHC protocol for MTHFD1 antibody 67670-1-IgDownload protocol
IF protocol for MTHFD1 antibody 67670-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.

Authors - Changhong Yang
View Article
humanWB

J Biol Chem

Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution

Authors - Yanjie Gao
  • KD Validated
View Article