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- KD/KO Validated
MPO Monoclonal antibody
MPO Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgA
Reactivity
human, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4C11F6
Cat no : 66177-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells |
| Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 14 publications below |
| IF | See 21 publications below |
| IP | See 1 publications below |
Product Information
66177-1-Ig targets MPO in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat, bovine, cow |
| Host / Isotype | Mouse / IgA |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MPO fusion protein Ag17564 相同性解析による交差性が予測される生物種 |
| Full Name | myeloperoxidase |
| Calculated molecular weight | 745 aa, 84 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC130476 |
| Gene symbol | MPO |
| Gene ID (NCBI) | 4353 |
| RRID | AB_2881572 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPO antibody 66177-1-Ig | Download protocol |
| IHC protocol for MPO antibody 66177-1-Ig | Download protocol |
| IF protocol for MPO antibody 66177-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Pathog Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice. | ||
Sci Rep Persisting and Increasing Neutrophil Infiltration Associates with Gastric Carcinogenesis and E-cadherin Downregulation. | ||
Sci Rep Interleukin 22 attenuated angiotensin II induced acute lung injury through inhibiting the apoptosis of pulmonary microvascular endothelial cells. | ||
Front Immunol Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow. | ||
Front Immunol An integrated bioinformatic investigation of mitochondrial energy metabolism genes in colon adenocarcinoma followed by preliminary validation of CPT2 in tumor immune infiltration | ||
Front Oncol Stromal Neutrophil Extracellular Trap Density Is an Independent Prognostic Factor for Cervical Cancer Recurrence. |
