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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using MPO Monoclonal antibody (66177-1-Ig)

    WB analysis of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66177-1-Ig (MPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using MPO Monoclonal antibody (66177-1-Ig)

    IF Staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using MPO Monoclonal antibody (66177-1-Ig)

    IF Staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HL-60 cells using MPO Monoclonal antibody (66177-1-Ig)

    IF Staining of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HL-60 cells using MPO Monoclonal antibody (66177-1-Ig)

    FC experiment of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    1x10^6 HL-60 cells were intracellularly stained with 0.25 ug MPO Monoclonal antibody (66177-1-Ig, Clone:4C11F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MPO Monoclonal antibody, PBS Only

MPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66177-1-PBS
Clone No.4C11F6

Host / Isotype

Mouse / IgA

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

4C11F6, EC:1.11.2.2, myeloperoxidase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Not recommend for IHC about rat sample. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66177-1-PBS targets MPO in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17564

Product name: Recombinant human MPO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 606-657 aa of BC130476

Sequence: CGLPQPETVGQLGTVLRNLKLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRV

相同性解析による交差性が予測される生物種
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC130476
Gene Symbol MPO
Gene ID (NCBI) 4353
RRIDAB_2881572
Conjugate Unconjugated
Form
FormLiquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP05164
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

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