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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human tonsillitis using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human tonsillitis using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human colon using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human colon using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    FC experiment of HeLa using Biotin-65161

    1x10^6 HeLa cells were surface stained with 0.5 ug Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161, Clone:6D4) or isotype control, and SA-PE. Cells were not fixed.

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Biotin Anti-Human MICA/MICB (6D4)

MICA/MICB Monoclonal Antibody for IHC, FC
Cat No. Biotin-65161
Clone No.6D4

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

IHC, FC

Synonyms

MICA, MIC-A, MIC A, 6D4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65161 targets MICA/MICB in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Transfected C1R cells expressing MICA 相同性解析による交差性が予測される生物種
Full Name MHC class I polypeptide-related sequence A
Calculated molecular weight 383 aa, 43 kDa
GenBank accession numberBC016929
Gene Symbol MICA
Gene ID (NCBI) 100507436
RRIDAB_2883067
Conjugate Biotin
Form Liquid
Purification MethodThe purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
UNIPROT IDQ29983
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

Protocols

Product Specific Protocols
IHC protocol for Biotin MICA/MICB antibody Biotin-65161Download protocol
FC protocol for Biotin MICA/MICB antibody Biotin-65161Download protocol
Standard Protocols
Click here to view our Standard Protocols