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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MEK1 Monoclonal antibody

MEK1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F6D7

Cat no : CL488-67872

Synonyms

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, PRKMK1

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL488-67872

    IF Staining of A431 using CL488-67872

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MEK1 antibody (CL488-67872, Clone: 3F6D7 ) at dilution of 1:200.

  • FC experiment of A431 using CL488-67872

    FC experiment of A431 using CL488-67872

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MEK1 (CL488-67872, Clone:3F6D7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67872-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67872 targets MEK1 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 1
Calculated molecular weight 43 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC139729
Gene symbol MAP2K1
Gene ID (NCBI) 5604
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MEK1 antibody CL488-67872Download protocol
FC protocol for CL Plus 488 MEK1 antibody CL488-67872Download protocol
Standard Protocols
Click here to view our Standard Protocols