MED23 Monoclonal antibody
MED23 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1H4G4
Cat no : 68307-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
68307-1-Ig targets MED23 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MED23 fusion protein Ag28483 相同性解析による交差性が予測される生物種 |
| Full Name | mediator complex subunit 23 |
| Calculated molecular weight | 156 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC060759 |
| Gene symbol | MED23 |
| Gene ID (NCBI) | 9439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED23 antibody 68307-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
