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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP1B Monoclonal antibody

MAP1B Monoclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6B9

Cat no : 67423-1-Ig

Synonyms

DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP 1B, MAP1B, MAP5

Validation Data Gallery

Applications:
  • IHC staining of rat brain using 67423-1-Ig

    IHC staining of rat brain using 67423-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 67423-1-Ig

    IHC staining of rat brain using 67423-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67423-1-Ig

    IHC staining of mouse brain using 67423-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 67423-1-Ig

    IF Staining of rat brain using 67423-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67423-1-Ig (MAP1B antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of SH-SY5Y using 67423-1-Ig

    FC experiment of SH-SY5Y using 67423-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human MAP1B (67423-1-Ig, Clone:1E6B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue
Positive FC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67423-1-Ig targets MAP1B in IF, FC, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAP1B fusion protein Ag17709 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 1B
Calculated molecular weight 2468 aa, 271 kDa
GenBank accession numberBC141853
Gene symbol MAP1B
Gene ID (NCBI) 4131
RRIDAB_2918490
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)

Protocols

Product Specific Protocols
IHC protocol for MAP1B antibody 67423-1-IgDownload protocol
IF protocol for MAP1B antibody 67423-1-IgDownload protocol
FC protocol for MAP1B antibody 67423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols