• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGT1 Polyclonal antibody

MAGT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27994-1-AP

Synonyms

bA217H1.1, DKFZp564K142, FLJ14726, IAG2, IAP, magnesium transporter 1, MAGT1, OST3B, PRO0756, UNQ628/PRO1244

Validation Data Gallery

Applications:
  • WB analysis using 27994-1-AP

    WB analysis using 27994-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27994-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 27994-1-AP

    WB analysis of NIH/3T3 using 27994-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27994-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HuH-7 using 27994-1-AP

    WB analysis of HuH-7 using 27994-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 27994-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells, HuH-7 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

27994-1-AP targets MAGT1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGT1 fusion protein Ag27416 相同性解析による交差性が予測される生物種
Full Name magnesium transporter 1
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC060842
Gene symbol MAGT1
Gene ID (NCBI) 84061
RRIDAB_2881033
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients' cells by western blot or immunofluorescent cell surface staining (Nature 475, 471-6). 27994-1-AP antibody detects the glycosylated protein around 45-50 kDa in SDS-PAGE. (PMID: 28720733, 27383987)

Protocols

Product Specific Protocols
WB protocol for MAGT1 antibody 27994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int J Mol Med

Transcription factor KLF16 activates MAGT1 to regulate the tumorigenesis and progression of breast cancer.

Authors - Lin Li
View Article