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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated LHCGR Polyclonal antibody

LHCGR Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-26424

Synonyms

FLJ41504, LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing hormone receptor

Validation Data Gallery

Applications:
  • IF Staining of mouse ovary using CL647-26424

    IF Staining of mouse ovary using CL647-26424

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite® Plus 647 LHCGR antibody (CL647-26424) at dilution of 1:200.

  • IF Staining of mouse ovary using CL647-26424

    IF Staining of mouse ovary using CL647-26424

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite® Plus 647 LHCGR antibody (CL647-26424) at dilution of 1:200.

  • FC experiment of Jurkat using CL647-26424

    FC experiment of Jurkat using CL647-26424

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human LHCGR (CL647-26424) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse ovary tissue
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 26424-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-26424 targets LHCGR in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LHCGR fusion protein Ag19259 相同性解析による交差性が予測される生物種
Full Name LHCGR
Calculated molecular weight 699 aa, 79 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC157028
Gene symbol LHCGR
Gene ID (NCBI) 3973
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 LHCGR antibody CL647-26424Download protocol
FC protocol for CL Plus 647 LHCGR antibody CL647-26424Download protocol
Standard Protocols
Click here to view our Standard Protocols