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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated LDHB Polyclonal antibody

LDHB Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14824

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL647-14824

    IF Staining of HeLa using CL647-14824

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 LDHB antibody (CL647-14824) at dilution of 1:100.

  • FC experiment of HeLa using CL647-14824

    FC experiment of HeLa using CL647-14824

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human LDHB (CL647-14824) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14824-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-14824 targets LDHB in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDHB fusion protein Ag6605 相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase B
Calculated molecular weight 36 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002362
Gene symbol LDHB
Gene ID (NCBI) 3945
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway (PMID: 26269128). It is glycolytic enzymes and it is positively regulated by mTOR.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 LDHB antibody CL647-14824Download protocol
FC protocol for CL Plus 647 LDHB antibody CL647-14824Download protocol
Standard Protocols
Click here to view our Standard Protocols