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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated LASP1 Monoclonal antibody

LASP1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G4B6

Cat no : CL488-68080

Synonyms

Lasp 1, LASP1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, MLN 50, MLN50

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-68080

    IF Staining of A549 using CL488-68080

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

  • IF Staining of A549 using CL488-68080

    IF Staining of A549 using CL488-68080

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

  • FC experiment of A549 using CL488-68080

    FC experiment of A549 using CL488-68080

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human LASP1 (CL488-68080, Clone:1G4B6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA549 cells
Positive FC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68080-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-68080 targets LASP1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LASP1 fusion protein Ag18101 相同性解析による交差性が予測される生物種
Full Name LIM and SH3 protein 1
Calculated molecular weight 30 kDa
Observed molecular weight35-38 kDa
GenBank accession numberBC012460
Gene symbol LASP1
Gene ID (NCBI) 3927
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 LASP1 antibody CL488-68080Download protocol
FC protocol for CL Plus 488 LASP1 antibody CL488-68080Download protocol
Standard Protocols
Click here to view our Standard Protocols